- 产品详情
- 产品参数
Product Name: Interleukin-10 (IL-10), Human, ELISA assay
Product Description : The human Interleukin 10 (IL-10) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-10 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-10 concentration are added to these wells and allowed to complex with the bound IL-10 antibody. A biotinylated human IL-10 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-10 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-10 in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative Names: Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10;
Application(s) : ELISA
Specificity: Human IL-10
Species Reactivity : Human
mmunogen Description : The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S19-N178
Range : 7.8 pg/mL - 500 pg/mL (cell culture supernates); 3.4 pg/mL - 250 pg/mL (human serum, plasma)
Sensitivity : < 0.5 pg/mL
Regulatory Status: For research use only.
