- 产品详情
- 产品参数
Product Name: Tumor necrosis factor alpha (TNF-alpha), Human, ELISA assay
Product Description :
Tumor necrosis factor alpha (TNF-alpha) is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF-alpha is mainly secreted by macrophages. TNF-alpha is involved in the regulation of many processes such as cell proliferation, differentiation and apoptosis and has been implicated in a variety of diseases including cancer. TNF-alpha is expressed as a single-pass type II membrane protein. A soluble, secreted form is derived from the membrane form by proteolytic processing.
The human TNF-alpha Kit is a sandwich ELISA. The capture antibody is a monoclonal human TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated human TNF-alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TNF-alpha in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative Names: TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;
Application(s): ELISA
Specificity: Human TNF-alpha
Species Reactivity: Human
Immunogen Description : The standard used in this human TNF alpha kit was also used as the antigen for the antibodies. It is a recombinant human protein fragment expressed in e. coli and purified. The exact sequence maps to the soluble form of TNF alpha, amino acids 77-233.
Range 15.6 pg/mL - 1,000 pg/mL (cell culture supernates), 7.8 pg/mL - 500 pg/mL (serum, plasma)
Sensitivity: < 1 pg/mL
Regulatory Status: For research use only.
