- 产品详情
- 产品参数
Product Name : | Interleukin-6 (IL-6), Human, ELISA assay |
Product Description | The human Interleukin 6 (IL-6) kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-6 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-6 concentration are added to these wells and allowed to complex with the bound IL-6 antibody. A biotinylated human IL-6 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-6 present in the samples. The purpose of this kit is for quantitative detection of human IL-6 in culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate) samples only when used as directed This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
Alternative Names | IL-6; Interleukin-6; B-cell stimulatory factor 2; BSF-2; Interferon beta-2; Hybridoma growth factor; CTL diffenrentiation factor; CDF; IL6; IFNB2; |
Application(s) | ELISA |
Specificity | Human IL-6 |
Species Reactivity | Human |
Immunogen Description | The standard product used in this kit is recombinant human IL-6, consisting amino acids 29-212 expressed in and purified from an E. coli expression system |
Range | 4.7 pg/mL - 300 pg/mL |
Sensitivity | < 0.3 pg/mL |
Regulatory Status | For research use only. |
