Product Name :
Interleukin-8 (IL-8), Human, ELISA assay
Product Description :
The human Interleukin 8 (IL-8) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-8 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-8 concentration are added to these wells and allowed to complex with the bound IL-8 antibody. A biotinylated human IL-8 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-8 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-8 in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative Names :
IL-8; Interleukin-8; C-X-C motif chemokine 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein; NAP-1; Protein 3-10C; T-cell chemotactic factor; IL8;
Application(s) : ELISA
Specificity Human: IL-8
Species Reactivity: Human
Immunogen Description:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S28-S99
Range :
15.6 pg/mL - 1,000 pg/mL (cell culture supernates); 7.8 pg/mL - 500 pg/mL (serum, plasma)
Sensitivity : < 1 pg/mL
Regulatory Status: For research use only.