WNT-5a, mouse

WNT-5a belongs to the class of WNT proteins that activate the “non-canonical” pathway.   The predicted size of mouse WNT-5a is a monomeric protein containing 356 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis under non-reducing conditions. StemRD’s product is expressed from a mouse cell line, and purified with a proprietary process that is distinct from the published method.

产品英文名称：WNT-5a,Mouse W5A-H-005,5ug
产品中文名称：小鼠 WNT-3a 蛋白因子，5ug

产品信息

Wnt糖蛋白是存在于多种生物体内的一种细胞外配体，Wnt作为形态发生素通过激发细胞内远离信号发送区域的浓度依赖反应控制胚胎形态发育。Wnt通路调节动物发育过程中的多个重要环节，例如细胞增殖、细胞迁徙及细胞分化。Wnt蛋白通过自分泌或旁分泌作用与位于细胞膜上的受体相结合，激活细胞内信号通路调节靶基因的表达，对细胞的增殖、分化、迁移、极性化和凋亡起到重要作用。

WNT-5a,MouseW5A-H-005,5ug 产品简介
wnt通路是细胞增殖分化的关键调控环节，在胚胎发育和肿瘤发生中起着重要作用。wnt途径参与了基因表达调节、细胞迁移粘附、细胞极化等过程，同时还与其它信号通路存在交叉协同。Wnt/β-catenin通路在进化过程中高度保守，此通路的主要分子构成及相关调控机制已得到基本阐明。对神经系统而言已有足够证据显示此通路参与了对神经前体细胞增殖，分化以及决定细胞命运的调控。
Wnt-3a干细胞生长分化因子是Wnt基因家族中重要成员，最近的一些研究发现， Wnt-3a信号蛋白对神经干细胞的定向分化的调控作用表现的十分明显。小鼠WNT-3a 干细胞生长分化因子蛋白包含328个氨基酸。
小鼠 WNT-3a 蛋白因子，5ug 产品特性
» 小鼠 WNT-3a 蛋白因子，5ug 干细胞生长分化因子活性经过NIH 3T3 细胞鉴定，纯度高
» 小鼠 WNT-3a 蛋白因子，5ug 干细胞生长分化因子生物活性在NIH 3T3 细胞经过TCF报告基因实验鉴定The EC50为 50–150 ng/ml
» 小鼠 WNT-3a 蛋白因子，5ug 干细胞生长分化因子产品经过SDS-PAGE 和HPLC双层鉴定纯度大于90%
WNT-5a,MouseW5A-H-005,5ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C,but should be stored in aliquots at -80 degree C for longer term. Avoid repeatedfreeze and thaw.
WNT-5a,MouseW5A-H-005,5ug 产品英文描述
WNT-3a is a member of the WNT family of signaling proteins that play a key rolein embryonic development and the integrity of adult tissues. WNT-5a,MouseW5A-H-005,5ug is a prototypic canonical WNT that signals through the β-cateninpathway. The predicted size of mouse WNT-5a,MouseW5A-H-005,5ug is a monomeric protein containing 333 amino acid residues. Due toglycosylation, it migrates at an apparent molecular weight of 40 kDa bySDS-PAGE analysis under nonreducing conditions. StemRD’s product WNT-5a,MouseW5A-H-005,5ug is expressed from a mouse cell line overexpressing mouse WNT-3a.Purification is performed with a proprietary process that is distinct from thepublished method.
•WNT-5a,Mouse W5A-H-005,5ug Purity: Greater than 90% as determined by SDS-PAGEanalysis

•WNT-5a,Mouse W5A-H-005,5ug Biological Activity: The activity was determined byusing a TCF reporter gene assay in NIH3T3 cells. The EC50 ranges from 50 - 150ng/ml. Country of Origin USA

References:

Ishida-Takagishi M, Enomoto A, et al., The Dishevelled-associating protein Daple controls the non-canonical Wnt/Rac pathway and cell motility. Nature Communication. 2012 May 29;3:859
http://www.ncbi.nlm.nih.gov/pubmed/22643886
Nakamuta S, Funahashi Y, Namba T et al., Local application of neurotrophins specifies axons through inositol 1,4,5-trisphosphate, calcium, and Ca2+/calmodulin-dependent protein kinases. Science Signal. 2011 Nov 15;4(199):ra76.
http://www.ncbi.nlm.nih.gov/pubmed/22087032